The size of the chromatographic column of Jiangyin Chuangtong Chromatography Equipment Co., Ltd. does not affect the degree of separation. The sample volume is large and the diameter of the column can be increased. Generally, the gel column used for preparation is larger than 2 cm in diameter, but it should be added during sample addition. The sample was evenly distributed on the bed of the gel column. In addition, the larger the diameter, the larger the elution volume, and the greater the dilution of the sample. The degree of separation depends on the height of the column. To separate the different components, the gel column bed must have a suitable height. The degree of separation is related to the square root of the column height, but the soft gel column is generally not more than 1 meter due to high extrusion deformation. .