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The column must have a suitable height

From:Jiangyin Chuangtong Laminate Equipment Co., Ltd.  Time:2018-5-15 8:24:56  Hits:

      Jiangyin Chuangtong Chromatography Equipment Co., Ltd. has a large amount of chromatographic column sample, which can increase the diameter of the column. Generally, a gel column with a diameter of more than 2 cm is prepared, but the sample should be evenly distributed on the gel column when adding the sample. On the bed. In addition, the larger the diameter, the larger the elution volume, and the greater the dilution of the sample. The degree of separation depends on the height of the column. To separate the different components, the gel column bed must have a suitable height. The degree of separation is related to the square root of the column height, but the soft gel column is generally not more than 1 meter due to high extrusion deformation.



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